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Vladislav Y. Orekhov group research

MDD and dynamics

Multi-Dimensional Decomposition (MDD) is a novel signal processing technique, which has been introduced and is being developed in the group. Distinct and documented features of the method include reliable deconvolution of overlapped signals in multidimensional NMR and ability to deal with optimally (i.e. non-uniformly) sampled data sets. As a result, much simplified spectra evaluation, i.e. defining peak positions and integrals, improved resolution and/or up to 80% saving of costly NMR measurement time are achieved for the NOESY type spectra, which carry majority of the structural information but also are among the most crowded and difficult for analysis.

In the field of structural proteomics the MDD is being implemented to boost efficiency of the NMR spectroscopy for protein structural studies; collaborations have been established (note, support by SSF - Genome Canada program) with the North-East-Structural Genomics Consortium in North America and the SPINE project in Europe (initial contacts). The superior spectral resolution provided by the MDD is demanded for large protein systems. For example, good results have been obtained for the four dimensional NOESY spectra of maltose binding protein (42 kDa) and malate synthase G (81.4 kDa). Studies of protein dynamics: methodology development and applications. Dynamics are critical for molecular function. In the case of biological macromolecules, important processes such as folding, ligand binding, electron transfer, enzyme catalysis and molecular recognition all involve motions that potentially span a wide range of time scales and amplitudes. The local developments and expertise in the group include major experimental and theoretical techniques in this field: model-free analysis for interpreting 15N, 13C or 2H relaxation data in terms of motions in the pico-nanosecond time scale; relaxation dispersion and T1rho experiments for assessing motions in milli-micro-second time scales; molecular dynamic simulations; hydrodynamics calculations for describing Brownian rotation of the molecules in solution. Applications include both globular (e.g. barnase, azurin) and membrane (e.g. bacteriorhodopsin) protein systems. Original software (DASHA) has been developed.

Contact Information

Vladislav Y. Orekhov

+46 (0)31 786 3886

+46 (0)31 786 3880

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Page Manager: Anders Pedersen|Last update: 8/30/2016

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